diff --git a/dom/canvas/test/webgl-conf/mochi-single.html b/dom/canvas/test/webgl-conf/mochi-single.html
index 894bdd7147e4..c909c144f942 100644
--- a/dom/canvas/test/webgl-conf/mochi-single.html
+++ b/dom/canvas/test/webgl-conf/mochi-single.html
@@ -68,7 +68,6 @@ function runTest() {
 }
 
 SpecialPowers.pushPrefEnv({"set": [
-  ['dom.workers.requestAnimationFrame', true],
   ['gfx.offscreencanvas.enabled', true],
   ['webgl.force-enabled', true],
   ['webgl.enable-draft-extensions', true],
diff --git a/dom/webidl/DedicatedWorkerGlobalScope.webidl b/dom/webidl/DedicatedWorkerGlobalScope.webidl
index 68c2c0fc640d..4b6b99a87e30 100644
--- a/dom/webidl/DedicatedWorkerGlobalScope.webidl
+++ b/dom/webidl/DedicatedWorkerGlobalScope.webidl
@@ -31,10 +31,10 @@ interface DedicatedWorkerGlobalScope : WorkerGlobalScope {
   // https://html.spec.whatwg.org/multipage/imagebitmap-and-animations.html#animation-frames
   // Ideally we would just include AnimationFrameProvider to add the interface,
   // but we cannot make an include conditional.
-  [Pref="dom.workers.requestAnimationFrame", Throws]
+  [Throws]
   long requestAnimationFrame(FrameRequestCallback callback);
 
-  [Pref="dom.workers.requestAnimationFrame", Throws]
+  [Throws]
   undefined cancelAnimationFrame(long handle);
 };
 
diff --git a/modules/libpref/init/StaticPrefList.yaml b/modules/libpref/init/StaticPrefList.yaml
index a10f6aa1e3ee..85a754e03d69 100644
--- a/modules/libpref/init/StaticPrefList.yaml
+++ b/modules/libpref/init/StaticPrefList.yaml
@@ -3910,11 +3910,6 @@
   value: true
   mirror: always
 
-- name: dom.workers.requestAnimationFrame
-  type: RelaxedAtomicBool
-  value: true
-  mirror: always
-
 - name: dom.workers.serialized-sab-access
   type: RelaxedAtomicBool
   value: false
diff --git a/testing/profiles/reftest/user.js b/testing/profiles/reftest/user.js
index 665fef8607b9..a5071fdf4549 100644
--- a/testing/profiles/reftest/user.js
+++ b/testing/profiles/reftest/user.js
@@ -104,4 +104,3 @@ user_pref("toolkit.legacyUserProfileCustomizations.stylesheets", true);
 user_pref("layout.css.prefers-color-scheme.content-override", 1);
 // Force OffscreenCanvas support
 user_pref("gfx.offscreencanvas.enabled", true);
-user_pref("dom.workers.requestAnimationFrame", true);
diff --git a/testing/profiles/web-platform/user.js b/testing/profiles/web-platform/user.js
index ca91b38393c7..f97cf99e9a9b 100644
--- a/testing/profiles/web-platform/user.js
+++ b/testing/profiles/web-platform/user.js
@@ -72,7 +72,6 @@ user_pref("media.block-autoplay-until-in-foreground", false);
 user_pref("layout.css.prefers-color-scheme.content-override", 1);
 // Force OffscreenCanvas support
 user_pref("gfx.offscreencanvas.enabled", true);
-user_pref("dom.workers.requestAnimationFrame", true);
 // A lot of tests use the Reporting API for observing things
 user_pref("dom.reporting.enabled", true);
 user_pref("layout.css.font-loading-api.workers.enabled", true);
diff --git a/testing/web-platform/meta/workers/WorkerGlobalScope_requestAnimationFrame.tentative.worker.js.ini b/testing/web-platform/meta/workers/WorkerGlobalScope_requestAnimationFrame.tentative.worker.js.ini
index 8e509f4fca1f..e70547876cee 100644
--- a/testing/web-platform/meta/workers/WorkerGlobalScope_requestAnimationFrame.tentative.worker.js.ini
+++ b/testing/web-platform/meta/workers/WorkerGlobalScope_requestAnimationFrame.tentative.worker.js.ini
@@ -1,4 +1,3 @@
 [WorkerGlobalScope_requestAnimationFrame.tentative.worker.html]
-  prefs: [dom.workers.requestAnimationFrame:true]
   expected:
     if (os == "android") and fission: [OK, TIMEOUT]